LOCUS       40924_41400        4419 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pSU7 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Nagano Y, Takao S, Kudo T, Iizasa E, Anai T.
  TITLE     Yeast-based recombineering of DNA fragments into plant 
            transformation vectors by one-step transformation
  JOURNAL   Plant Cell Rep. 26 (12), 2111-2117 (2007)
  PUBMED    17680244
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Nagano Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2006) Yukio Nagano, Saga University, Analytical 
            Research Center for Experimental Sciences; Honjo-machi 1, Saga 
            840-8502, Japan (E-mail:nagano@cc.saga-u.ac.jp, Tel:81-952-28-8898, 
            Fax:81-952-28-8896)
REFERENCE   3  (bases 1 to 4419)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4419)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Rep."; date: "2007"; volume: "26"; issue: "12"; pages: "2111-2117"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JAN-2006) Yukio Nagano, Saga University, Analytical Research 
            Center for Experimental Sciences"; volume: " Honjo-machi 1, Saga 
            840-8502, Japan (E-mail:nagano@cc.saga-u.ac.jp, Tel:81-952-28-8898, 
            Fax"; pages: "81-952-28-8896"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4419
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             205..996
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      1063..1943
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        2542..2762
                     /label=URA3 promoter
     CDS             2763..3563
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      3722..4310
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggatccatcg gaattcatcc gcggtttcaa aatcggctcc gtcgatacta tgttatacgc
       61 caactttgaa aacaactttg aaaaagctgt tttctggtat ttaaggtttt agaatgcaag
      121 gaacagtgaa ttggagttcg tcttgttata attagcttct tggggtatct ttaaatactg
      181 tagaaaagag gaaggaaata ataaatggct aaaatgagaa tatcaccgga attgaaaaaa
      241 ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc taaggtatat
      301 aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg gtataaaggg
      361 accacctatg atgtggaacg ggaaaaggac atgatgctat ggctggaagg aaagctgcct
      421 gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct catgagtgag
      481 gccgatggcg tcctttgctc ggaagagtat gaagatgaac aaagccctga aaagattatc
      541 gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga ttgtccctat
      601 acgaatagct tagacagccg cttagccgaa ttggattact tactgaataa cgatctggcc
      661 gatgtggatt gcgaaaactg ggaagaagac actccattta aagatccgcg cgagctgtat
      721 gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg cgacctggga
      781 gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct tgggagaagc
      841 ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc
      901 ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag
      961 aaaataaaat attatatttt actggatgaa ttgttttagt acctagaatg aatcttcctg
     1021 tttttggggc ttttctgatt atcaaccggg gtggagcttc ccattgcgaa taccgcttcc
     1081 acaaacattg ctcaaaagta tctctttgct atatatctct gtgctatatc cctatataac
     1141 ctacccatcc acctttcgct ccttgaactt gcatctaaac tcgacctcta cattttttat
     1201 gtttatctct agtattactc tttagacaaa aaaattgtag taagaactat tcatagagtg
     1261 aatcgaaaac aatacgaaaa tgtaaacatt tcctatacgt agtatataga gacaaaatag
     1321 aagaaaccgt tcataatttt ctgaccaatg aagaatcatc aacgctatca ctttctgttc
     1381 acaaagtatg cgcaatccac atcggtatag aatataatcg gggatgcctt tatcttgaaa
     1441 aaatgcaccc gcagcttcgc tagtaatcag taaacgcggg aagtggagtc aggctttttt
     1501 tatggaagag aaaatagaca ccaaagtagc cttcttctaa ccttaacgga cctacagtgc
     1561 aaaaagttat caagagactg cattatagag cgcacaaagg agaaaaaaag taatctaaga
     1621 tgctttgtta gaaaaatagc gctctcggga tgcatttttg tagaacaaaa aagaagtata
     1681 gattctttgt tggtaaaata gcgctctcgc gttgcatttc tgttctgtaa aaatgcagct
     1741 cagattcttt gtttgaaaaa ttagcgctct cgtcgcgttg catttttgtt ttacaaaaat
     1801 gaagcacaga ttcttcgttg gtaaaatagc gctttcgcgt tgcatttctg ttctgtaaaa
     1861 atgcagctca gattctttgt ttgaaaaatt agcgctctcg cgttgcattt ttgttctaca
     1921 aaatgaagca cagatgcttc gttaacaaag atatgctatt gaagtgcaag atggaaacgc
     1981 agaaaatgaa ccggggatgc gacgtgcaag attacctatg caatagatgc aatagtttct
     2041 ccaggaaccg aaatacatac attgtcttcc gtaaagcgct agactatata ttattataca
     2101 ggttcaaata tactatctgt ttcagggaaa actcccaggt tcggatgttc aaaattcaat
     2161 gatgggtaac aagtacgatc gtaaatctgt aaaacagttt gtcggatatt aggctgtatc
     2221 tcctcaaagc gtattcgaat atcattgaga agctgcagcg tcacatcgga taataatgat
     2281 ggcagccatt gtagaagtgc cttttgcatt tctagtctct ttctcggtct agctagtttt
     2341 actacatcgc gaagatagaa tcttagatca cactgccttt gctgagctgg atcaatagag
     2401 taacaaaaga gtggtaaggc ctcgttaaag gacaaggacc tgagcggaag tgtatcgtac
     2461 agtagacgga gtatctagta tagtctatag tccgtggaat taattctcat ctttgacagc
     2521 ttatcatcga taagctagct tttcaattca attcatcatt ttttttttat tctttttttt
     2581 gatttcggtt tctttgaaat ttttttgatt cggtaatctc cgaacagaag gaagaacgaa
     2641 ggaaggagca cagacttaga ttggtatata tacgcatatg tagtgttgaa gaaacatgaa
     2701 attgcccagt attcttaacc caactgcaca gaacaaaaac ctgcaggaaa cgaagataaa
     2761 tcatgtcgaa agctacatat aaggaacgtg ctgctactca tcctagtcct gttgctgcca
     2821 agctatttaa tatcatgcac gaaaagcaaa caaacttgtg tgcttcattg gatgttcgta
     2881 ccaccaagga attactggag ttagttgaag cattaggtcc caaaatttgt ttactaaaaa
     2941 cacatgtgga tatcttgact gatttttcca tggagggcac agttaagccg ctaaaggcat
     3001 tatccgccaa gtacaatttt ttactcttcg aagacagaaa atttgctgac attggtaata
     3061 cagtcaaatt gcagtactct gcgggtgtat acagaatagc agaatgggca gacattacga
     3121 atgcacacgg tgtggtgggc ccaggtattg ttagcggttt gaagcaggcg gcagaagaag
     3181 taacaaagga acctagaggc cttttgatgt tagcagaatt gtcatgcaag ggctccctat
     3241 ctactggaga atatactaag ggtactgttg acattgcgaa gagcgacaaa gattttgtta
     3301 tcggctttat tgctcaaaga gacatgggtg gaagagatga aggttacgat tggttgatta
     3361 tgacacccgg tgtgggttta gatgacaagg gagacgcatt gggtcaacag tatagaaccg
     3421 tggatgatgt ggtctctaca ggatctgaca ttattattgt tggaagagga ctatttgcaa
     3481 agggaaggga tgctaaggta gagggtgaac gttacagaaa agcaggctgg gaagcatatt
     3541 tgagaagatg cggccagcaa aactaaaaaa ctgtattata agtaaatgca tgtatactaa
     3601 actcacaaat tagagcttca atttaattat atcagttatt acccctcatg accaaaatcc
     3661 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     3721 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     3781 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3841 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     3901 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3961 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     4021 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     4081 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     4141 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     4201 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     4261 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     4321 acgcggcctt tttacggttc ctgggctttt gctggccttt tgctcacatg ttctttcctg
     4381 cgttatcccc tgattctgtg gataaccgta ttaccgccg
//
